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anti rat cd10 antibody  (R&D Systems)


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    R&D Systems anti rat cd10 antibody
    Knockout of STING attenuates, but does not negate, inflammatory gene up-regulation induced by double-stranded DNA (dsDNA) in tubule epithelial cells (TECs). A: Dual staining of primary cultured TECs for aquaporin 1 (AQP1; red) and <t>CD10</t> (green). B: Immunoblotting kidney homogenates of wild-type and STING KO mice showing absence of STING protein in STING KO mice. C: Acrylamide gel showing dsDNA after preparation according to the protocol described previously, and 1 to 3 are replicates. D: Immunoblotting for STING, cGMP-AMP synthase (cGAS), TANK-binding kinase 1 (TBK1) Ser172 phosphorylation (pTBK1), total TBK1, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in control and STING KO TECs transfected with vehicle or 2.5 μg dsDNA for 6 hours E – I: Quantitative real-time RT-PCR for Ifnb1 ( E ), Il6 ( F ), Tnf ( G ), Tgfb1 ( H ), and Havcr1 ( I ) in TECs transfected with vehicle or dsDNA. Values are means ± SD ( D – I ). n = 6 per condition ( D – I ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001 by one-way analysis of variance with Tukey post test. Scale bars = 50 μm ( A ).
    Anti Rat Cd10 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neprilysin/Human%2FMouse+Neprilysin%2FCD10+Antibody/pmc12881290-86-8-20
    Average 92 stars, based on 7 article reviews
    anti rat cd10 antibody - by Bioz Stars, 2026-10
    92/100 stars

    Images

    1) Product Images from "Stimulator of Interferon Genes Is a Fine-Tuner, but Not a Prime Mover, of Kidney Inflammation"

    Article Title: Stimulator of Interferon Genes Is a Fine-Tuner, but Not a Prime Mover, of Kidney Inflammation

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2025.10.013

    Knockout of STING attenuates, but does not negate, inflammatory gene up-regulation induced by double-stranded DNA (dsDNA) in tubule epithelial cells (TECs). A: Dual staining of primary cultured TECs for aquaporin 1 (AQP1; red) and CD10 (green). B: Immunoblotting kidney homogenates of wild-type and STING KO mice showing absence of STING protein in STING KO mice. C: Acrylamide gel showing dsDNA after preparation according to the protocol described previously, and 1 to 3 are replicates. D: Immunoblotting for STING, cGMP-AMP synthase (cGAS), TANK-binding kinase 1 (TBK1) Ser172 phosphorylation (pTBK1), total TBK1, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in control and STING KO TECs transfected with vehicle or 2.5 μg dsDNA for 6 hours E – I: Quantitative real-time RT-PCR for Ifnb1 ( E ), Il6 ( F ), Tnf ( G ), Tgfb1 ( H ), and Havcr1 ( I ) in TECs transfected with vehicle or dsDNA. Values are means ± SD ( D – I ). n = 6 per condition ( D – I ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001 by one-way analysis of variance with Tukey post test. Scale bars = 50 μm ( A ).
    Figure Legend Snippet: Knockout of STING attenuates, but does not negate, inflammatory gene up-regulation induced by double-stranded DNA (dsDNA) in tubule epithelial cells (TECs). A: Dual staining of primary cultured TECs for aquaporin 1 (AQP1; red) and CD10 (green). B: Immunoblotting kidney homogenates of wild-type and STING KO mice showing absence of STING protein in STING KO mice. C: Acrylamide gel showing dsDNA after preparation according to the protocol described previously, and 1 to 3 are replicates. D: Immunoblotting for STING, cGMP-AMP synthase (cGAS), TANK-binding kinase 1 (TBK1) Ser172 phosphorylation (pTBK1), total TBK1, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in control and STING KO TECs transfected with vehicle or 2.5 μg dsDNA for 6 hours E – I: Quantitative real-time RT-PCR for Ifnb1 ( E ), Il6 ( F ), Tnf ( G ), Tgfb1 ( H ), and Havcr1 ( I ) in TECs transfected with vehicle or dsDNA. Values are means ± SD ( D – I ). n = 6 per condition ( D – I ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001 by one-way analysis of variance with Tukey post test. Scale bars = 50 μm ( A ).

    Techniques Used: Knock-Out, Staining, Cell Culture, Western Blot, Acrylamide Gel Assay, Binding Assay, Phospho-proteomics, Control, Transfection, Quantitative RT-PCR

    Related Articles

    In Vitro:

    Article Title: Discovery of O -glycans on atrial natriuretic peptide (ANP) that affect both its proteolytic degradation and potency at its cognate receptor
    Article Snippet: .. In vitro cleavage activity was assayed by adding 1 ng of neprilysin (R&D Systems) or 50 ng of insulin-degrading enzyme (R&D Systems) to 325 pmol of peptide or glycopeptide substrate in a total volume of 10 μl. .. Data processing was performed using Proteome Discoverer version 1.4 software (Thermo Fisher Scientific), using Sequest HT node as described previously with small changes ( 22 ).

    Activity Assay:

    Article Title: Discovery of O -glycans on atrial natriuretic peptide (ANP) that affect both its proteolytic degradation and potency at its cognate receptor
    Article Snippet: .. In vitro cleavage activity was assayed by adding 1 ng of neprilysin (R&D Systems) or 50 ng of insulin-degrading enzyme (R&D Systems) to 325 pmol of peptide or glycopeptide substrate in a total volume of 10 μl. .. Data processing was performed using Proteome Discoverer version 1.4 software (Thermo Fisher Scientific), using Sequest HT node as described previously with small changes ( 22 ).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Novel and established biomarkers to complement risk scores in patients with acute decompensated heart failure – a pilot study
    Article Snippet: .. Novel cardiorenal biomarkers were measured in urine including neutrophil gelatinase-associated lipocalin (uNGAL) (Human Lipocalin-2/NGAL ELISA, BioVendor, Eching, Germany), Neprilysin (Human Neprilysin DuoSet ELISA, R&D Systems Minneapolis, USA), Dickkopf-3 (DKK3) (Human DKK3 DuoSet ELISA, R&D Systems Minneapolis, USA) and Interleukin-6 (IL-6) (ECLIA, Roche Diagnostics Mannheim, Germany) and in serum, including cystatin C (immunoturbidimetric assay, Roche Diagnostics Mannheim, Germany) NGAL, Neprilysin (Human Neprilysin DuoSet ELISA, R&D Systems Minneapolis, USA), Growth Differentiation Factor 15 (GDF-15) (Human GDF-15 DuoSet ELISA, R&D Systems Minneapolis, USA), soluble Suppression of Tumorgenicity 2 (sST2) (Human sST2/IL-33R DuoSet ELISA R&D Systems, Minneapolis, USA), Galectin 3 (Human Galectin 3 DuoSet ELISA, R&D Systems Minneapolis, USA) and Progranulin (Human Progranulin DuoSet ELISA, R&D Systems Minneapolis, USA). .. Enzyme linked immunosorbent assays (ELISA) were performed in accordance with instructions supplied by the manufacturer.

    Article Title: Novel and established biomarkers to complement risk scores in patients with acute decompensated heart failure - a pilot study.
    Article Snippet: Study Objective: There are several risk scores for mortality in patients with acute decompensated heart failure (ADHF) such as the European Collaboration on Acute Decompensated Heart Failure Score (ELAN-HF Score), the ADHF/NT-proBNP-Score or A2B-Score (age, anemia, BNP).. The aim of this study was to evaluate the predictive value of such risk scores with and without addition of novel cardiorenal biomarkers.. Design & Setting: Single-center, exploratory prospective cohort study at the University Hospital Heart Centre Brandenburg.

    Immunoturbidimetry Assay:

    Article Title: Novel and established biomarkers to complement risk scores in patients with acute decompensated heart failure – a pilot study
    Article Snippet: .. Novel cardiorenal biomarkers were measured in urine including neutrophil gelatinase-associated lipocalin (uNGAL) (Human Lipocalin-2/NGAL ELISA, BioVendor, Eching, Germany), Neprilysin (Human Neprilysin DuoSet ELISA, R&D Systems Minneapolis, USA), Dickkopf-3 (DKK3) (Human DKK3 DuoSet ELISA, R&D Systems Minneapolis, USA) and Interleukin-6 (IL-6) (ECLIA, Roche Diagnostics Mannheim, Germany) and in serum, including cystatin C (immunoturbidimetric assay, Roche Diagnostics Mannheim, Germany) NGAL, Neprilysin (Human Neprilysin DuoSet ELISA, R&D Systems Minneapolis, USA), Growth Differentiation Factor 15 (GDF-15) (Human GDF-15 DuoSet ELISA, R&D Systems Minneapolis, USA), soluble Suppression of Tumorgenicity 2 (sST2) (Human sST2/IL-33R DuoSet ELISA R&D Systems, Minneapolis, USA), Galectin 3 (Human Galectin 3 DuoSet ELISA, R&D Systems Minneapolis, USA) and Progranulin (Human Progranulin DuoSet ELISA, R&D Systems Minneapolis, USA). .. Enzyme linked immunosorbent assays (ELISA) were performed in accordance with instructions supplied by the manufacturer.

    Article Title: Novel and established biomarkers to complement risk scores in patients with acute decompensated heart failure - a pilot study.
    Article Snippet: Study Objective: There are several risk scores for mortality in patients with acute decompensated heart failure (ADHF) such as the European Collaboration on Acute Decompensated Heart Failure Score (ELAN-HF Score), the ADHF/NT-proBNP-Score or A2B-Score (age, anemia, BNP).. The aim of this study was to evaluate the predictive value of such risk scores with and without addition of novel cardiorenal biomarkers.. Design & Setting: Single-center, exploratory prospective cohort study at the University Hospital Heart Centre Brandenburg.

    Incubation:

    Article Title: NXP031 restores memory function by dual effects degrading Aβ accumulation and facilitating antioxidant pathway in Alzheimer's disease models.
    Article Snippet: Alzheimer’s disease (AD) is a representative neurodegenerative disease that is characterized by the overaccumulation of amyloid beta (Aβ) proteins.. Since AD is accompanied by excessive oxidative stress, which aggravates neurological pathologies, the use of antioxidants has been considered to prevent disease development.. NXP031, a combination of vitamin C (VitC) and an optimized aptamer that binds to VitC and stabilizes the reactivity of VitC, was designed.

    Recombinant:

    Article Title: Substrate selective inhibitors of insulin-degrading enzyme (IDE) and uses thereof
    Article Snippet: .. The proteases IDE42-1019, recombinant human IDE42-1019 (R&D Systems), neprilysin (R&D), and angiotensin-converting enzyme (R&D) were assayed using the fluorophore/quencher-tagged peptide substrate Mca-RPPGFSAFK(Dnp)-OH (SEQ ID NO: 24) (R&D) according to the manufacturer's instructions and using the recommended buffers (fluorophore Mca=(7-methoxycourmarin-4-yl)acetyl and quencher Dnp=2,4-dinitrophenyl). .. The proteases IDE42-1019, recombinant human IDE42-1019 (R&D Systems), neprilysin (R&D), and angiotensin-converting enzyme (R&D) were assayed using the fluorophore/quencher-tagged peptide substrate Mca-RPPGFSAFK(Dnp)-OH (SEQ ID NO: 24) (R&D) according to the manufacturer's instructions and using the recommended buffers (fluorophore Mca=(7-methoxycourmarin-4-yl)acetyl and quencher Dnp=2,4-dinitrophenyl).

    Article Title: Enhancers of neurolysin activity
    Article Snippet: .. Human recombinant peptidases—recombinant human neurolysin (product number 3814-ZN), thimet oligopeptidase (TOP; product number 3439-ZN), neprilysin (NEP; product number 1182-ZNC), angiotensin converting enzyme (ACE; product number 929-ZN), and ACE2 (product number 933-ZN) were purchased from R & D systems. ..



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    Image Search Results


    Knockout of STING attenuates, but does not negate, inflammatory gene up-regulation induced by double-stranded DNA (dsDNA) in tubule epithelial cells (TECs). A: Dual staining of primary cultured TECs for aquaporin 1 (AQP1; red) and CD10 (green). B: Immunoblotting kidney homogenates of wild-type and STING KO mice showing absence of STING protein in STING KO mice. C: Acrylamide gel showing dsDNA after preparation according to the protocol described previously, and 1 to 3 are replicates. D: Immunoblotting for STING, cGMP-AMP synthase (cGAS), TANK-binding kinase 1 (TBK1) Ser172 phosphorylation (pTBK1), total TBK1, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in control and STING KO TECs transfected with vehicle or 2.5 μg dsDNA for 6 hours E – I: Quantitative real-time RT-PCR for Ifnb1 ( E ), Il6 ( F ), Tnf ( G ), Tgfb1 ( H ), and Havcr1 ( I ) in TECs transfected with vehicle or dsDNA. Values are means ± SD ( D – I ). n = 6 per condition ( D – I ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001 by one-way analysis of variance with Tukey post test. Scale bars = 50 μm ( A ).

    Journal: The American Journal of Pathology

    Article Title: Stimulator of Interferon Genes Is a Fine-Tuner, but Not a Prime Mover, of Kidney Inflammation

    doi: 10.1016/j.ajpath.2025.10.013

    Figure Lengend Snippet: Knockout of STING attenuates, but does not negate, inflammatory gene up-regulation induced by double-stranded DNA (dsDNA) in tubule epithelial cells (TECs). A: Dual staining of primary cultured TECs for aquaporin 1 (AQP1; red) and CD10 (green). B: Immunoblotting kidney homogenates of wild-type and STING KO mice showing absence of STING protein in STING KO mice. C: Acrylamide gel showing dsDNA after preparation according to the protocol described previously, and 1 to 3 are replicates. D: Immunoblotting for STING, cGMP-AMP synthase (cGAS), TANK-binding kinase 1 (TBK1) Ser172 phosphorylation (pTBK1), total TBK1, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in control and STING KO TECs transfected with vehicle or 2.5 μg dsDNA for 6 hours E – I: Quantitative real-time RT-PCR for Ifnb1 ( E ), Il6 ( F ), Tnf ( G ), Tgfb1 ( H ), and Havcr1 ( I ) in TECs transfected with vehicle or dsDNA. Values are means ± SD ( D – I ). n = 6 per condition ( D – I ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001 by one-way analysis of variance with Tukey post test. Scale bars = 50 μm ( A ).

    Article Snippet: For CD10 immunostaining, cells were stained with monoclonal anti-rat CD10 antibody at 1:200 dilution (catalog number MAB1126; lot number IMO0321021; R&D Systems, Minneapolis, MN) and Alexa Fluor 488 donkey anti-rat secondary antibody at 1:750 dilution (catalog number a-21208; lot number 1701951; Thermo Fisher Scientific).

    Techniques: Knock-Out, Staining, Cell Culture, Western Blot, Acrylamide Gel Assay, Binding Assay, Phospho-proteomics, Control, Transfection, Quantitative RT-PCR